Effect of resveratrol on cell viability and expression of SIRT1 and DBC1 in cultured rat GCs. (A) Effect of resveratrol on cell viability at 72 h was estimated by MTS assay. Results are shown as the mean percentage of the untreated control ± SEM (bars) of eight wells of three independent experiments * p < 0.05 vs. control. ** p < 0.01 vs. control. (B and C) Effect of resveratrol on mRNA levels of (B) SIRT1 and (C) DBC1 was investigated by quantitative real-time RT-PCR. The mRNA level of the untreated control was arbitrarily set at 1.0, and that of the treatment group was estimated relative to the control value. Results are shown as the mean ± SEM (bars) of three independent experiments. ** p < 0.01 vs. control. (D) Effect of resveratrol on protein levels of SIRT1 was investigated by Western blot. Resveratrol treatment resulted in an increased expression of SIRT1 protein, and the results were consistent with that of quantitative real time RT-PCR. Three independent experiments were performed and a representative result is shown.