Effects of administration of BMP-6 on ovarian expressions of Id-1 and VEGF. Eighteen hours after hCG injection, both whole ovaries of each mouse were collected, and the expressions of ovarian Id-1 and VEGF were examined by RT-PCR and western blot. (A) Representative RT-PCR for mRNA expression of ovarian Id-1 and VEGF. Levels of mRNA for Id-1 and VEGF were normalized to the amount of GAPDH per sample. (B) Representative western blot products of protein expression for ovarian Id-1 and VEGF. Levels of protein for Id-1 and VEGF were normalized to the amount of actin per sample. Data are representative of at least three independent experiments. The relative density of each gene was quantified with NIH-Image J program (version 1.35d) (*P < 0.05 and **P < 0.01).