Relative BMP-15 levels in poor responders. A: Western blotting analysis of the BMP-15 protein in follicular fluid. The BMP-15 in 0.4 μl of FF following SDS gel electrophoresis, transfer and blotting on a nitrocellulose membrane with a polyclonal antibody. B: Comparison of the relative levels of BMP-15 in follicular fluid by western blotting analysis in poor responders. The level of follicular fluid BMP-15 in the high BMP-15 group was significantly higher than that in the low BMP-15 group (**P < 0.001).