Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

Table 3 Detection rate (DR) and false positive (FP) rate in each analyte combination for prospective study (PS) and retrospective study (RS)

Study type	No. Down’s	A	B	C	Additive effect on A’
Study type	No. Down’s	PAPP-A hCGbeta	PAPP-A sLHCGR	hCGbeta sLHCGR	Additive effect on A’
PS 350 + 13	N = 13
	DR%	46.1	84.6	53.84	38.46
	FP%	1.7	4.6	6	4.6
RS 120 + 30	N = 30
	DR%	56.6	46.7	50	13.3
	FP%	0.83	4.16	13.3	4.16
Total 470 + 43	N = 43
	DR%	53.5	58.13	51.1	20.9
	FP%	1.5	4.46	7.9	4.46

The cut-off values used for PAPP-A, hCGbeta and hCG-sLHCGR were ≤0.5 MoM, ≥2.0 MoM and ≤5.0 plus ≥170 pmol/mL, respectively. Additive Effect on A (PAPP-A and hCGbeta measurement) shows the percentage of additional T21 pregnancies detected by the hCG-sLHCGR and PAPP-A combination which had not been detected by PAPP-A and hCGbeta measurements.

ISSN: 1477-7827