Skip to main content
Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

Figure 3

The sensitivity and relationship of the sLHCGR and hCGbeta-sLHCGR ELISA assays. The recombinant LHCGR and yoked hCG -LHCGR proteins together with anti-LHCGR and anti-hCG mono- and polyclonal antibodies provide quantitative standard curves in ELISA; a) and b), the coomassie-stained affinity purified recombinant human a) LHCGR ECD and b) hCGbeta-LHCGR proteins resolved in SDS-PAGE; c and d, both serially diluted protein standard c) and three serum samples from first trimester pregnancy d) exhibited linear response in ELISA assays where hCG-LHCGR complex was captured by 5A10C9 and was detected by HRP-conjugated anti-hCGbeta monoclonal antibody (clone 094–10627, Acris, Germany): e), shows strong positive correlation (r = 0.88) between sLHCGR and hCG-LHCGR when the analytes were measured in the same set of serum samples (N, 179).

Back to article page