Skip to main content

Table 1 Primer sequences and the conditions of RT-PCR

From: Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplement

Primer

Sequence (5’ → 3’)

Product size (bp)

Annealing temperature (°C)

Cycles

GAPDH

FW- TGATGACATCAAGAAGGTGGTGAAG

230

57

30

 

REV -TCCTTGGAGGCCATGTGGGCCAT

   

C-KIT

FW- TTGTGCACGACGATGTCTGA

296

57

30

 

REV- AGTCCATACCTCCCTCTCTT

   

DAZL

FW- GAGTGGTCAAAGGAGCCAAA

144

57

30

 

REV- AGGAGCCACCTCCCTGAG

   

VASA

FW- TCTTCACAAGCTCCCAATCC

165

57

30

 

REV- TGAGAATACAAGGACAGGAGCT

   

ZP3

FW- GCTTTGCCTTCTGACACCTC

127

57

30

 

REV- CAGACACAGGGTGGGAGG

   

DMC1

FW- AGCAGCAAAGTTCCATGAAG

300

56

30

 

REV- TGAGCTCTCCTCTTCCCTTT

   

SCP3

FWD-TGGAAAACACAACAAGATCA

344

56

30

 

REV-GCTATCTCTTGCTGCTGAGT

   

STRA8

FW-CCTCAAAGTGGCAGGTTCTGAA

126

56

30

 

REV-TCCTCTAAGCTGCTTGCATGC

  Â