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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplement

Figure 5

Gene and protein expression of meiosis specific markers. RT-PCR of DMC1, SCP3 and STRA8 gene expression in freshly isolated hAECs and in hAECs cultured in medium containing SSS at passage 3 and passage 5. Photographs of representative bands of the gene products in a 2.5% agarose gel from four independent experiments are shown. GAPDH gene expression was used as an internal control. (B and C) Immunofluorescence staining of hAECs grown in medium containing SSS at passage 5, (B) nuclear and cytoplasmic DMC1 expression (red); (C) cytoplasmic SCP3 (red). On the left side are bright field micrograph of the same cells, scale bar = 10μm; magnification x100. Blue color, DAPI nuclear staining.

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