Figure 3From: Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplementImmunofluorescence staining of hAECs negative for DAZL, ZP3 and GDF9. (A) Freshly isolated hAECs; (B) hAECs cultured in medium containing SSS at passage 1 and at passage 3 (C). hAECs cultured in standard medium at passage 1 (D), at passage 3 (E) and at passage 5 (F). Blue color, DAPI nuclear staining of the corresponding cells.Back to article page