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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis

Figure 2

Electrophoretic Mobility Shift Assays (EMSA) for testicular HIF-1 binding. The DNA-binding activity of testicular HIF-1 was determined in EMSA experiments. (A) Results of positive control experiment using biotin-labelled oligonucleotides for Epstein-Barr nuclear antigen (EBNA), a positive control protein extract for EBNA (Ext), competition with unlabeled EBNA oligonucleotides, and competition with non-specific oligonucleotides for β-actin. (B) Representative EMSA results for cytoplasmic or nuclear (C) protein extracts from normoxic testes incubated with biotin-labelled HIF-1 oligonucleotides containing the consensus HRE. Actin lanes contain a molar excess of unlabeled β-actin oligonucleotides to demonstrate the specificity of protein binding to the HIF-1 oligonucleotides.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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