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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis

Figure 1

Testicular HIF-1 displays DNA-binding activity by ELISA. Nuclear protein extracts from ischemic (I) testes, sham-treated (S) testes, and testes subjected to ischemia-reperfusion (I/R) demonstrating in vitro binding to the HIF HRE in ELISA experiments. Absorbance readings were quantitated as the fold increase over negative control values. COS-7 protein extracts were included as positive controls for HIF-1 DNA binding. The HIF-1 binding in experimental samples was significantly higher than that in negative control samples (*ANOVA, p<0.05), but there were no statistically significant differences in binding activity among the experimental samples.

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