Figure 3

Percentage of methylation of HOXA11 , region II in infertile women with endometriosis (E1-18), fertile women (C1-16) and infertile women with tubal occlusion (S1-S16). Eutopic mid-luteal endometrium samples were used for genomic DNA isolation followed by bisulfite conversion of cytosine bases to uracil. The HOXA11 II region was then amplified by a pair of primers complementary to the bisulfite-DNA modified sequence (Aditional file 1, Table S1; Aditional file 2, Figure S1). The PCR products were purified with subsequent cloning into a plasmid vector. Plasmid DNA isolated from ten positive bacterial clones was used for commercial sequencing. The results of bisulphite sequencing were assessed and presented using BiQ analyzer software [31] and BDPC web server [32]^aMann-Whitney Rank Sum Test.