Figure 2

DNMT1 (A), DNMT3A (B) and DNMT3B (C) transcript levels in eutopic mid-luteal endometrium from infertile women with endometriosis, fertile women and infertile women with tubal occlusion. The eutopic endometrial tissue from women with endometriosis, fertile women and infertile women with tubal occlusion was respectively obtained by pipelle or hysteroscopic biopsy during the implantation window, followed by total RNA isolation. RNA was reverse-transcribed and DNMT1, DNMT3A and DNMT3B cDNAs were investigated by RQ-PCR relative quantification analysis. To normalize the quantity of transcripts in each sample, DNMTs mRNA levels were normalised to the amount of GAPDH and ACTB cDNA. The amounts of DNMTs mRNA were expressed as the multiplicity of these cDNA copies in the calibrator. The boxes and the middle lines correspond to the values from the lower to upper quartiles and medians, respectively.