Figure 8

In situ hybridization of BMPR-IB mRNA in ovaries of adult cycling rats. Brightfield (A, C, D, E, F, G, H, J, L, O) and Darkfield (B, I, K, M, N). A, B: Sections from a P 2000 h ovary (4X); positive GC in dominant (DF), AF and preantral follicle (arrow head); Note positive oocyte. C: Positive sex cords at P 1000 h (40X);D: Artery at P 2000 h (40X) showing positive tunica adventita (TA) next to smooth muscle (SM); E: Positive oocytes (O) in primordial and early primary follicles at DI 1100 h (40X); F: Positive O in late primary follicle with single layer of weakly positive GC at P 2000 h (40X); G: Strongly positive O and GC in secondary follicle at E 1000 h (20X); H, I: Higher magnification (20X) of wall of DF at P 1000 h showing positive GC, TE, and a number of TIC in the outer zone of TI; J, K: Positive AF at P 1000 h (10X); L, M: CL-I at DII 1100 h showing positive TE (arrow heads); N: CL-I at P 2000 h showing positive TE at periphery (arrow head) and clusters of positive cells scattered within the CL (); O: Higher magnification (40X) of N showing positive endothelial (EC) and a few adjacent weakly positive granulosa lutein cells (arrow head).