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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: The spatiotemporal expression pattern of the bone morphogenetic protein family in rat ovary cell types during the estrous cycle

Figure 6

In situ hybridization of BMP-15 mRNA in ovaries of adult cycling rats. Brightfield (A, C, D, E, F, G) and Darkfield (B). A, B: Sections from a DII 1100 h ovary (4X); Positive oocytes in preantral follicles (arrow heads); C: Early primary follicle at DI 1100 h showing one cuboidal GC and a weakly positive oocyte: note most of the signal is located within the GV or nucleus (40X). D: Preantral follicle at P 1000 h (40X) in the process of forming a second layer of GC. Note a weakly positive oocyte; E: Mid secondary follicle at DII 1100 h showing a strongly positive full-grown oocyte surrounded by three to four layers of GC (20X); F: Mid cross section of a strongly positive oocyte of a dominant follicle at P 1000 h (40X). Hybridization signals are seen predominantly in the ooplasm, with some signal associated with the outer region of the nucleolus (arrow head); G: An atretic follicle at E 0200 h (40X) with a moderately positive oocyte that has resumed meiosis

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