Figure 3

TIMP-2 mRNA and protein expression in the bovine CL. A) In the upper panel, Northern blotting of TIMP-2 mRNA in early (E), mid (M), and late (L) stage bovine CL. The arrows indicate the 3.5 and 1 kb species. The lower panel is the same membrane hybridized with a human cyclophilin probe. B) Densitometric ratios of each TIMP-2 mRNA band to cyclophilin in corresponding stages are shown. Dissimilar letters denote significant difference at p < 0.05. C) A representative Western blot of TIMP-2 in the bovine CL. Molecular masses (kDa) of protein standards are shown on the left. A single 22 kDa TIMP-2 immunoreactive protein band, indicated by the arrow, is present in early (E), mid (M), and late (L) stage CL. D) Densitometric analysis of TIMP-2 protein expression among different stages of CL. Dissimilar letters denote significant difference at p < 0.05.