Generating and manipulating transgenic animals using transposable elements

Table 1 Transposable elements active in vertebrate species for use as gene transfer and insertional mutagenesis vectors.

Transposon	Family^a	Activity in cultured cells^b	Activity in vertebrate animals^c	References
L1	LINE retrotransposon	Mouse (PCT) Human (PCT)	Mouse (TCRG)	[21, 22]
Tol2	hAT	Mouse (EP) Human (EP)	Medaka (TCRG) Zebrafish (TCRG).	[27, 28, 32]
Tc1	Tc1/mariner	Human (PCT) Mouse (PCT)	-	[19, 39, 40]
Tc3	Tc1/mariner	Human (PCT)	Zebrafish (GT and TCRG)	[19, 42]
Mariner (Himar1)	Tc1/mariner	Human (IPT)	-	[19, 44]
Mariner (Mos1)	Tc1/mariner	Human (PCT)	Chicken (GT), zebrafish (GT)	[5, 19, 43]
Minos	Tc1/mariner	Human (PCT)	Mouse (TCRG) Mouse (TCRS)	[46–48]
Sleeping Beauty	Tc1/mariner	Mouse, hamster, human, monkey, dog, cow, sheep, quail, Xenopus, many fish (PCT)	Mouse (GT) Mouse lung, liver (SCT) Mouse (TCRG) Mouse (TCRS)	[4, 19, 30, 51, 53–56, 59, 60]

^aRelevant superfamily of transposable element. ^bActivity in immortalized cell lines has been demonstrated using by demonstrating excision from an introduced plasmid (EP), interplasmid transposition (IPT), or full transposition from introduced plasmid (or viral vector) into chromosomes (PCT). ^cActivity in intact animals (various species) has been demonstrated by germline transgenesis (GT), somatic cell transgenesis (SCT), transposition of chromosomally resident transposon vectors in the germline (TCRG), and transposition of chromosomally resident transposon vectors in the soma (TCRS).

ISSN: 1477-7827