Figure 5
Three-dimensional projection showing an ovulated unfertilized metaphase II zona intact oocyte collected 14 hours after the hCG injection and stained with ABL2 and LCA. arrowhead = equator; asterisk = cortical granule free domain; arrow = perivitelline space. ZP = indicates the zona pellucida which does not actually show as it is not fluorescently labeled. Entire oocyte was optically sectioned, and all optical sections were used to compile the 3D projection, which was animated. A movie of this oocyte can be viewed by double clicking the movie file Fig5Movie.avi ("see Additional file 1").