Figure 2

In situ hybridization and immunohistochemical analysis of COX-2 expression in the endometrium of cyclic ewes. Cross-sections of ovine endometrium were hybridized with radiolabeled antisense or sense bovine COX-2 cRNA probes. Hybridized sections were digested with ribonuclease A, and protected transcripts were visualized by liquid emulsion autoradiography. Developed slides were counterstained lightly with hematoxylin, and photomicrographs were taken under bright-field (left) or dark-field illumination (middle). Immunoreactive COX-2 protein was detected using rabbit anti-human COX-2 polyclonal IgG (right). The negative IgG control was performed by substituting irrelevant rabbit IgG for primary antibody. The white arrow denotes areas of specific COX-2 mRNA or immunoreactive protein expression. Legend: C, cyclic; LE, lumenal epithelium; S, stroma; sGE, superficial ductal glandular epithelium. Bar = 20 μm.