Immunohistochemical staining of proximal efferent ductules on day 125 post treatment. A,B. ERα is expressed abundantly in control efferent ductule epithelium, with slightly less staining in the ciliated cells (apical nuclei), but is reduced in the ICI-treated tissue. C,D. ERβ is also abundant in efferent ductule epithelial cells, both in the controls and treated samples. E,F. Sodium/hydrogen exchanger (NHE3) is stained heavily along the apical border of the nonciliated cells of control tissue, but is absent in the ICI-treated tissue. G,H. Aquaporin-1 (AQP-1) is abundant along the microvillus border of the control ductules, but is diminished in the treated tissue, although intensely stained along the apical border of some cells. I,J. Carbonic anhydrase II (CAII) is present along the microvillus border and in apical cytoplasm of control ductules, but is greatly reduced in the epithelium of treated tissues. Bar = 25 μm.