Figure 4

Mean (± sed) number of viable theca cells after 144 hours in serum free culture in (A) the presence of 100 ng/ml LR3 IGF-1, 0.01 ng/ml LH and 10 ng/ml insulin (control – light bars), supplemented with oocyte conditioned medium (OCM) (hatched bars) and OCM supplementation without LR3 IGF-1 (dark bars); (B) in the presence of either 0, 5, 10 or 15 oocytes per well supplemented with 100 ng/ml LR3 IGF-1, 0.01 ng/ml LH and 10 ng/ml insulin. Values are from 3 independent cultures, each treatment having 4 replicates. There was a significant (P = 0.02) effect of OCM on viable theca cell number. There was a significant effect of oocyte number per well on viable cell numbers (P = 0.029). Bars with different superscripts are significantly different (P < 0.05). Gross morphology of porcine theca cells co-cultured with oocytes. C and D represent theca cells supplemented with 5 oocytes after 144 hours in culture and (D) stained with neutral red. Scale bar represents 100 μm.