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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: SPAM1 (PH-20) protein and mRNA expression in the epididymides of humans and macaques: utilizing laser microdissection/RT-PCR

Figure 4

A). Western blot analysis of protein extracts from testis, epididymis, vas deferens and sperm of human (Subject #2) and macaque #1. Equal amounts (40 μg) of protein were loaded in each lane. The arrow points to the ~64 kDa SPAM1 band in all samples. This experiment was repeated three times. A 53 kDa band is also seen for human sperm, while the epididymis shows weaker bands (partially degraded products) at ~60 kDa. In both species the vas deferens show a weak <50 kDa band. B) Dot blot analysis of protein extracts from macaque testis, caput, corpus, and cauda. Bovine serum albumin (BSA) was used as a negative control. Equal volumes with approximately equal amounts of protein (40 μg) were blotted for each tissue.

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