IHC of CDC47/MCM7, Akt and Phospho-Akt in response to 17β-estradiol (E2) in rat endometrium. Animals were ovariectomized for at least 10 days and then injected daily with E2 (or vehicle for control group) for 3 days. Rats were killed and their uteri collected, fixed and sectioned for IHC. IHC shown are from one representative experiment and were repeated five times using five different uterine sections from five different rats per day of estrous cycle. Arrow indicates strong redistribution of Phospho-Akt at the membrane level. LE = luminal epithelium, S = stroma.