Figure 6
Expression of CDC47/MCM7 (A), Akt (B) and Phospho-Akt (C) in response to 17β-estradiol (E2) in rat endometrium. Animals were ovariectomized for at least 10 days and then injected daily with E2 (or vehicle for control group) for 3 days. Rats were killed and endometrial proteins were collected for Western analysis. β-actin blots shown were used as controls to correct for loading in each lane. Blots shown are from one representative experiment. Graphics represent western blots densitometrical analysis and are the mean ± SEM of five independent experiments (5 different rats/endometrial extract per treatment group). *Significantly different from control (p < 0.001).