Proliferation and apoptosis in the rat endometrium during the estrous cycle as demonstrated by Western analyses. Rats were killed at different days of estrous cycle (diestrus, proestrus, estrus and metestrus) and total endometrial proteins were collected. A) Proliferation as determined by Western analysis of CDC47/MCM7. B) Apoptosis as determined by Western analysis of intact and cleaved caspase-3. Data represent the mean ± SEM of six independent experiments for CDC47/MCM7 and five for intact and cleaved caspase-3. β-actin blots shown were used as controls to correct for loading in each lane. Blots shown are from one representative experiment. Graphics represent Western blots densitometrical analysis. Columns with different superscript are significantly different (different letters are different from each other) (p < 0.05).