Figure 4

Granulosa cell expression of A) IL-1β and B) IL-1RA mRNA at different stages: early dominance [ED], end of growth [T0 h], 6 h [T6 h], 12 h [T12 h], 24 h [T24 h] and 34 h [T34 h] after injection of crude equine gonadotropin. The relative amount of each PCR product isolated by electrophoresis was obtained by scanning each band. Gene expression intensity (arbitrary unit) was measured by ImageQuant software and is expressed as an intensity value normalized to the gapdh intensity value. The results are expressed in arbitrary units and represent means ± SEM of seven to ten samples. a and b are significantly different (p < 0.05 at least), a and c tended to be different (p < 0.06), b and c are not different according to the non parametric Wilcoxon Mann Whitney test. The data presented are representative of two experiments (samples collection, RNA extraction and PCR).