Figure 3

Gapdh, IL-1β, IL-1RA and IL-1R2 gene expression in equine granulosa cells at different stages. Lane M, molecular weight markers (100-bp DNA ladder, Promega); lane P, positive control of PCR (cDNA from lipopolysaccharide-stimulated equine macrophages); lane N, negative control of PCR (no cDNA); lane 1, early dominance [ED]; lane 2, end of growth [T0 h]; lanes 3, 4, 5 and 6, 6 h [T6 h], 12 h [T12 h], 24 h [T24 h], and 34 h [T34 h] after injection of crude equine gonadotropin, respectively. The expected sizes of amplified fragments are indicated.