Gelatine-agarose affinity profile of goat seminal plasma proteins. Ten mg of goat seminal plasma proteins were dissolved in 2 ml of PB and loaded on the column (1 × 20 cm). The unadsorbed proteins were removed by washing successively with PB, PBS and 0.5 M urea in PBS. Five M urea in PBS was then used to elute the gelatin-binding proteins. Four ml fractions were collected at a flow rate of 30 ml/h and pooled as indicated (fraction A and B). Proteins were monitored at 280 nm.