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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Placental expression of estrogen receptor beta and its hormone binding variant – comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells

Figure 4

Immunolocalization of ERβ and ERα in the late trophoblast culture (68 h). [A] Isolated CT cells are unstained (arrowhead) but strong ERβ nuclear and cytoplasmic immunostaining accompanies young ST aggregates (y). [B] CT cell associated with ST shows ERβ immunoreactivity (arrowhead). [C] Mature ST (m) shows a diminution of ERβ nuclear staining (solid > white arrows) with persisting cytoplasmic immunoreactivity. [D] Secondary antibody control. [E] Isolated CT cells show strong ERα immunostaining (arrowhead) persisting in young ST aggregates [y, E and F] but diminishing in mature ST [m; F and G]. [H] Secondary antibody control.

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