Figure 1

Photomicrographs representing examples of immunostaining for cytokeratin, IL-11 and IL-11Rα in cynomolgus monkey implantation sites at days 12, 14 and 17 of pregnancy. Positive cells are shown as brown pigment with hematoxylin counterstain. (A) Cytokeratin staining at day 14 gestation showing chorionic villi (v), cytotrophoblast shell (s,) and epithelial plaque cells (p); (B and C) Day 12 gestation showing villi and shell. (B) IL-11 staining; (C) IL-11Rα staining. (D and E) Day 17 gestation showing shell and decidua; (D) IL-11 staining, decidua (d); (E) IL-11Rα staining. (F) IL-11Rα staining at day 14 gestation showing epithelial plaque cells. Insert represents negative control. (G-M) Day 17 gestation: (G) cytokeratin staining identifying trophoblast cells in: the shell, invaded artery (ina), and stroma (st); (H) IL-11 staining; arrow indicates positively stained trophoblast cells of invaded artery; (I) IL-11Rα staining; arrow indicates positively stained trophoblast cells of invaded artery; (J) IL-11 staining showing decidua, insert represents negative control; (K) IL-11Rα staining showing gland (g) and decidua; (L) IL-11 staining showing spiral artery at centre; (M) IL-11Rα staining showing spiral arteries. Scale bar represents 50 μm.