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Table 1 4-AP Effects on Granulosa Cells Cultured in Defined Serum-free Media

From: 4-Aminopyridine Decreases Progesterone Production by Porcine Granulosa Cells

Variable Treatment Day 0 Day 1 Day 2
Media Progesterone Accumulation (ng/ml) (n = 12) CONTROL 50.7 ± 6.9 314.9 ± 45.7 519.7 ± 87.7
  4-AP 40.2 ± 4.8 46.8 ± 3.9a 25.2 ± 4.3a
  FSH 43.0 ± 7.0 653.5 ± 86.1 623.2 ± 95.3
  FSH + 4-AP 52.9 ± 8.0 206.8 ± 55.9b 101.0 ± 26.8b
Number of Viable Cells (cells/well × 105) (n = 3) CONTROL 2.7 ± 0.2 7.4 ± 1.9 6.3 ± 0.7
  4-AP 3.4 ± 1.3 2.2 ± 0.8a 2.2 ± 0.3a
  FSH 2.8 ± 0.5 5.5 ± 0.1 5.6 ± 0.0
  FSH + 4-AP 4.1 ± 1.1 3.8 ± 0.2b 3.0 ± 0.2b
  1. a Signficantly different from control on same day post-treatment. b Signficantly different from FSH on same day post-treatment.