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Table 1 4-AP Effects on Granulosa Cells Cultured in Defined Serum-free Media

From: 4-Aminopyridine Decreases Progesterone Production by Porcine Granulosa Cells

Variable

Treatment

Day 0

Day 1

Day 2

Media Progesterone Accumulation (ng/ml) (n = 12)

CONTROL

50.7 ± 6.9

314.9 ± 45.7

519.7 ± 87.7

 

4-AP

40.2 ± 4.8

46.8 ± 3.9a

25.2 ± 4.3a

 

FSH

43.0 ± 7.0

653.5 ± 86.1

623.2 ± 95.3

 

FSH + 4-AP

52.9 ± 8.0

206.8 ± 55.9b

101.0 ± 26.8b

Number of Viable Cells (cells/well × 105) (n = 3)

CONTROL

2.7 ± 0.2

7.4 ± 1.9

6.3 ± 0.7

 

4-AP

3.4 ± 1.3

2.2 ± 0.8a

2.2 ± 0.3a

 

FSH

2.8 ± 0.5

5.5 ± 0.1

5.6 ± 0.0

 

FSH + 4-AP

4.1 ± 1.1

3.8 ± 0.2b

3.0 ± 0.2b

  1. a Signficantly different from control on same day post-treatment. b Signficantly different from FSH on same day post-treatment.