4-AP increases granulosa cell size. Dot plots of forward scatter (SIZE FSC) vs. propidium iodide fluorescence (PI FL-2) for granulosa cells cultured in either the absence (CON) or presence of 4-AP (2 mM). Each dot represents one cell. Dead cells stained with propidium iodide appear in two upper quadrants. Cellular debris and shrunken apoptotic cells are displayed in the lower left quadrant. Viable granulosa cells are represented in the lower right quadrant. Median forward scatter was 637 and 735 for viable CON and 4-AP, respectively.