Skip to main content

Table 1 TGF-β activity in lysates of isolated testicular cells (Tissue) and in cell culture.

From: Rat testicular germ cells and sertoli cells release different types of bioactive transforming growth factor beta in vitro

  TGF-β activity in pg × 10-6 cells/24 h
  Tissue Supernatant
Testicular cell type Not activated Heat activated Not activated Heat activated
Spermatocytes ND 5.0 ± 0.6 693.0 ± 231.0 6405.0 ± 105.0
Round spermatids ND 1.5 ± 0.2 361.4 ± 100.6 2537.5 ± 262.5
Elongating spermatids ND 103.1 ± 9.4 596.3 ± 138.4 2981.3 ± 393.8
Sertoli cells ND 16.7 ± 6.7 233.3 ± 66.7 1458.3 ± 208.3
  1. Supernatant, using the CCl64 mink lung epithelial cell line as a bioassay for the measurement of TGF-β activity. Spermatocytes, round spermatids, elongated spermatids and Sertoli cells were cultured for 24 h. TGF-β activity in the different preparations was measured before and after heat-activation of the samples (for details see Materials & Methods). The incubations were carried out in triplicate (values are presented as means ± SD); the data being representative of at least three different experiments. ND: not detectable.