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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Oolemmal proteomics – identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane

Figure 2

2D Electrophoresis, biotinylation and avidin blotting identifies surface-labeled oolemmal proteins. 2D electrophoresis of 800 zona-free mouse egg proteins, surface labeled with 2 mg/ml Sulfo-NHS biotin, and separated by 2D electrophoresis. Blotted with streptavidin-HRP and visualized by ECL (A). Corresponding 2D electrophoretic gel of 800 unlabeled zona-free mouse eggs visualized by silver staining. Labelled black arrows indicate previously identified protein spots (B). Horizontal axis of each shows isoelectric point and vertical axis shows molecular weight (kDa).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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