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Table 1 Primer and probe sequences used for real-time RT-PCR

From: Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization

Target Primer Sequence Reporting Dye Product Size(bp)
SAHH Forward 5'-TGTTGCTTTTATGTCTCTCTGG-3' SYBR Green 154
  Reverse 5'-GCTTGGCATTCTCTTAAACC-3'   
HSC70 Forward 5'-GTCTTCCTTGCTCAAACG-3' SYBR Green 164
  Reverse 5'-AACTCACAGGCATACCTCC-3'   
OSU-T1-50 Forward 5'-TCACGGTTAGTGTCGCATGA-3' 6-FAM-CGTGTTTCCTACGTTGGGCGTCC-TAMRA 72
  Reverse 5'-ACCCATGAACAGGTCCTGAA-3'   
  1. Primer and probe sequences used for real-time RT-PCR, reporting dye used for detection of amplified product and product size for s-adenosylhomocysteine hydrolase (SAHH), heat shock cognate 70 kDa (HSC70) and the novel OSU-T1-50.