Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization

Table 1 Primer and probe sequences used for real-time RT-PCR

Target	Primer Sequence	Reporting Dye	Product Size(bp)
SAHH	Forward 5'-TGTTGCTTTTATGTCTCTCTGG-3'	SYBR Green	154
	Reverse 5'-GCTTGGCATTCTCTTAAACC-3'
HSC70	Forward 5'-GTCTTCCTTGCTCAAACG-3'	SYBR Green	164
	Reverse 5'-AACTCACAGGCATACCTCC-3'
OSU-T1-50	Forward 5'-TCACGGTTAGTGTCGCATGA-3'	6-FAM-CGTGTTTCCTACGTTGGGCGTCC-TAMRA	72
	Reverse 5'-ACCCATGAACAGGTCCTGAA-3'

Primer and probe sequences used for real-time RT-PCR, reporting dye used for detection of amplified product and product size for s-adenosylhomocysteine hydrolase (SAHH), heat shock cognate 70 kDa (HSC70) and the novel OSU-T1-50.

ISSN: 1477-7827