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Table 2 PCR schedule

From: Expression of the insulin-like growth factor (IGF) system and steroidogenic enzymes in canine testis tumors

Primer

Temperature

Cycles

Magnesium

Gel %

Aro

MJ 55°C

35

1.5 mM

1.2

AR

PE 55°C

30

1.5 mM

1.2

IGF-I

PE55°C

35

1.5 mM

1.2

IGF-II

PE55°C

35

1.5 mM

1.2

IGF-IR

PE58→50°C

35

1.5 mM

1.2

Red 1

55°C

35

3.0 mM

1.0

Red 2

55°C

35

3.0 mM

1.5

IGFBP1

58→50°C

35

1.5 mM

1.5

IGFBP2

60→55°C

35

1.5 mM

1.2

IGFBP3

55°C

35

4.0 mM

1.5

IGFBP4

55°C

30

1.5 mM

1.2

IGFBP5

55°C

30

1.5 mM

1.2

  1. Aro = p450-aromatase enzyme, AR = androgen receptor, IGF = insulin-like growth factor, IR = receptor type I, Red 1 = type I 5α-reductase enzyme, Red 2 = type II 5 α reductase enzyme, IGFBP = binding protein, MJ = MJ biozym thermal cycler, PE = Perkin Elmer Cetus.
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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