|
Primer
|
Temperature
|
Cycles
|
Magnesium
|
Gel %
|
|---|
|
Aro
|
MJ 55°C
|
35
|
1.5 mM
|
1.2
|
|
AR
|
PE 55°C
|
30
|
1.5 mM
|
1.2
|
|
IGF-I
|
PE55°C
|
35
|
1.5 mM
|
1.2
|
|
IGF-II
|
PE55°C
|
35
|
1.5 mM
|
1.2
|
|
IGF-IR
|
PE58→50°C
|
35
|
1.5 mM
|
1.2
|
|
Red 1
|
55°C
|
35
|
3.0 mM
|
1.0
|
|
Red 2
|
55°C
|
35
|
3.0 mM
|
1.5
|
|
IGFBP1
|
58→50°C
|
35
|
1.5 mM
|
1.5
|
|
IGFBP2
|
60→55°C
|
35
|
1.5 mM
|
1.2
|
|
IGFBP3
|
55°C
|
35
|
4.0 mM
|
1.5
|
|
IGFBP4
|
55°C
|
30
|
1.5 mM
|
1.2
|
|
IGFBP5
|
55°C
|
30
|
1.5 mM
|
1.2
|
- Aro = p450-aromatase enzyme, AR = androgen receptor, IGF = insulin-like growth factor, IR = receptor type I, Red 1 = type I 5α-reductase enzyme, Red 2 = type II 5 α reductase enzyme, IGFBP = binding protein, MJ = MJ biozym thermal cycler, PE = Perkin Elmer Cetus.
