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Table 2 PCR schedule

From: Expression of the insulin-like growth factor (IGF) system and steroidogenic enzymes in canine testis tumors

Primer Temperature Cycles Magnesium Gel %
Aro MJ 55°C 35 1.5 mM 1.2
AR PE 55°C 30 1.5 mM 1.2
IGF-I PE55°C 35 1.5 mM 1.2
IGF-II PE55°C 35 1.5 mM 1.2
IGF-IR PE58→50°C 35 1.5 mM 1.2
Red 1 55°C 35 3.0 mM 1.0
Red 2 55°C 35 3.0 mM 1.5
IGFBP1 58→50°C 35 1.5 mM 1.5
IGFBP2 60→55°C 35 1.5 mM 1.2
IGFBP3 55°C 35 4.0 mM 1.5
IGFBP4 55°C 30 1.5 mM 1.2
IGFBP5 55°C 30 1.5 mM 1.2
  1. Aro = p450-aromatase enzyme, AR = androgen receptor, IGF = insulin-like growth factor, IR = receptor type I, Red 1 = type I 5α-reductase enzyme, Red 2 = type II 5 α reductase enzyme, IGFBP = binding protein, MJ = MJ biozym thermal cycler, PE = Perkin Elmer Cetus.