Figure 1

The whole testicular extracts of immature porcine testes contain both sst2A receptor protein (A) and transcript (B). (A) Western blot analysis of sst2A receptor immunoreactivity in soluble protein extracts (100 μg) separated by 10 % SDS-PAGE. After the electrotransfer of proteins, the corresponding nitrocellulose membrane was divided into three parts. Two parts were immunoblotted with either R57 antibody alone (first lane: "immune") or in the presence of the antigenic peptide (second lane: "preadsorbed"). The third part was incubated in parallel with the preimmune serum (third lane: "preimmune"). Molecular mass (in kDa) is indicated by the arrow. (B) Amplification products obtained in PCR reaction with sst2-specific primers. Templates used in the reaction are: cDNA corresponding to the whole testicular tissue (lane "T+") and cultured Leydig cells (lane "L+") obtained in the RT reactions as described in Materials and Methods; mRNAs obtained from the whole testicular tissue (lane "T-") and cultured Leydig cells (lane "L-") and processed according to the same RT protocol as the former samples but without the addition of MMLV. The size (in bp) of DNA fragments is given by the lane "M". The lower panel shows the amplification of internal standard GAPDH-fragments in the corresponding samples.