Figure 7
From: The envelopes of amphibian oocytes: physiological modifications in Bufo arenarum
Binding of CGP to VE , CE , FE and sperm. Proteins of VE , CE , FE and sperm were resolved on 7.5 % SDS-PAGE and transferred to nitrocellulose. The membrane was incubated with biotin-labeled CGP and reveled with streptavidine peroxidase by chemoluminiscence. Components of CGP bind to gp120, gp41 and gp38 but not to gp75. No evidence of binding to sperm was observed. A, binding of labeled CGP ; B, negative controls; C, PAS-stained proteins. Images are representative of three independent experiments.