TNFα induces loss of viability of CLENDO cells in vitro. (Panel A) CLENDO cells were treated for 48 h with media alone (Ctl), TNFα (50 ng/ml), IFNγ (200 IU/ml), Jo2 (500 ng/ml), or PGF2α (1 μM). Cells were stained with Hoechst 33258 and the numbers of non-apoptotic cells per field in each of 10 separate fields per dish were counted. (Panel B) CLENDO cells were treated for 72 h with media alone (control), soluble FasL (50 ng/ml), human Fas activating antibody (CH-11, 500 ng/ml) or bovine IFNγ (200 IU/ml). Cell viability was determined as described above. These data represent the mean ± SEM of at least three independent experiments. The ''*'' denotes significance (P < 0.05) compared to untreated control.