Responsiveness of bovine cumulus-oocyte-complexes (COC) to porcine and recombinant human FSH, and the effect of COC quality on gonadotropin receptor and Cx43 marker gene mRNAs during maturation in vitro

Table 2 The effect of varying concentrations of pFSH with and without serum, and other hormones on cumulus expansion.

Hormone (ng/ml)	Percentage of Expanded COCs (%)¹
100 pFSH	9.1 ± 3.3^a
100 pFSH + 10% serum	55.3 ± 11.1^ab
1000 pFSH	72.6 ± 10.8^b
1000 pFSH, 5000 pLH, 1000 estradiol and 10% serum (FLES)	90.3 ± 2.5^b

¹COCs that were evaluated as Category II and III at 21–22 h of maturation were considered expanded. Data are Means ± SEM. ^a,b denotes differences between treatments, P < 0.05. Data are from eight replicates with 750–800 COCs per treatment.

ISSN: 1477-7827