Figure 7From: Retracted Article : Gonadotropin releasing hormone analogue (GnRHa) alters the expression and activation of Smad in human endometrial epithelial and stromal cellsThe effect of GnRH (0.1 μM) on Smad3 activation in HES and ESC pretreated with TGF-β type II receptor antisense (1 μM) or sense (1 μM) oligonucleotides for 24 hrs. The cell lysates were prepared from GnRH-treated (+) and untreated (-) cells after 5, 15 and 30 min and analyzed by immunoblotting for pSmad2/3 and β-actin as loading control, shown from a representative experiment. Bar graph shows the mean ± SEM of fold change in the rate of Smad3 activation from three independent experiments with denotes ** are significantly different from untreated (*) controls (p < 0.05).Back to article page