The extracellular matrix of porcine mature oocytes: Origin, composition and presumptive roles

Table 3 in vitro MATURATION and in vivo FERTILIZATION of PIG OOCYTES CULTURED in MEDIUM with TUNICAMYCIN and ³H-GLUCOSAMINE

Time of culture for in vitro maturation	Culture conditions (1)		No of oocytes used	No of oocytes (2) in			Oocytes (3) fertilized showing
	³H-glucosamine	Tunicamycin		GV	MI	MII	syngamy	3–4 pronuclei
0 – 40 hr (GV MII)		T	55	2 [>5]	10 [18]	43 [78]
	G		8				6 [75]	2 [25]
	G	T	10				8 [80]	2 [20]
16 or 20 – 40 hr (LD MII)		T	48	0 [0]	4 [8]	44 [>91]
	G		10				9 [90]	1 [10]
	G	T	12				6 [50]	6 [50]

(1) Pig oocytes isolated 72 hr after PMSG injection (0 hr) or 16–20 hr after hCG injection were cultured in the modified TC 199 supplemented with (G) or without ³H-glucosamine in presence (T) or absence of Tunicamycin. (2) At the end of the culture, cumulus cells were removed, oocytes were mounted on slides, fixed and stained with aceto-orcein. (3) In four experiments, the cultured oocytes were transferred into the oviducts of mated unilaterally ovariectomized gilts and flushed out 16–18 hr after transfer. All eggs were evaluated on semi-thin sections. [] = percent

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