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Table 3 in vitro MATURATION and in vivo FERTILIZATION of PIG OOCYTES CULTURED in MEDIUM with TUNICAMYCIN and 3H-GLUCOSAMINE

From: The extracellular matrix of porcine mature oocytes: Origin, composition and presumptive roles

Time of culture for in vitro maturation Culture conditions (1) No of oocytes used No of oocytes (2) in Oocytes (3) fertilized showing
  3H-glucosamine Tunicamycin   GV MI MII syngamy 3–4 pronuclei
0 – 40 hr (GV MII)   T 55 2 [>5] 10 [18] 43 [78]   
  G   8     6 [75] 2 [25]
  G T 10     8 [80] 2 [20]
16 or 20 – 40 hr (LD MII)   T 48 0 [0] 4 [8] 44 [>91]   
  G   10     9 [90] 1 [10]
  G T 12     6 [50] 6 [50]
  1. (1) Pig oocytes isolated 72 hr after PMSG injection (0 hr) or 16–20 hr after hCG injection were cultured in the modified TC 199 supplemented with (G) or without 3H-glucosamine in presence (T) or absence of Tunicamycin. (2) At the end of the culture, cumulus cells were removed, oocytes were mounted on slides, fixed and stained with aceto-orcein. (3) In four experiments, the cultured oocytes were transferred into the oviducts of mated unilaterally ovariectomized gilts and flushed out 16–18 hr after transfer. All eggs were evaluated on semi-thin sections. [] = percent