Skip to main content

Table 2 The EFFECT of TUNICAMYCIN on 3H-GLUCOSAMINE INCORPORATION in cumulus, ooplasm and PVS, as observed on semi-thin sections

From: The extracellular matrix of porcine mature oocytes: Origin, composition and presumptive roles

    Labeling (2)
Oocyte stage at onset
of experiment
Duration
of culture (hr)
Culture
medium (1)
Cumulus
cells
ooplasm PVS
GV (0 hr post hCG) 1   ++ + +
  1+3CH   ++ + +
  20   ++++ ++ +++
  20+24CH   +++ + +++
  40    ++ +++
  40 (H)    + +
  40 T   ++ ++++
  40 (H) T   + +
LD (20 hrs post hCG) 1   ++ + +
  1+3CH   ++ + ++
  24    ++ +++
  24 (H)    + +
  24 T   ++ ++++
  24 (H) T   + +
  1. (1) The modified TC 199 was supplemented with 3H-glucosamine in thepresence (T) or absence of Tunicamycin (2) Same scale of labeling as inTable 1 CH = chase (H) = hyaluronidase treatment at the end of culture