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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: The extracellular matrix of porcine mature oocytes: Origin, composition and presumptive roles

Figure 5

Effect of Tunicamycin and hyaluronidase on continuous incorporation of 3H-glucosamine from GVBD stage until MII after initiation of maturation in vivo (a, b, c, d) or in vitro (e, f, g, h). bar = 10 μm a : labeling of corona cells (C), of the surface of the ZP (Z), of the PVS (*) (accumulation) and of the ooplasm in a control oocyte.b : hyaluronidase treatment removes most of the labeling.c : after Tunicamycin treatment, labeling is accumulated in the PVS (*), around the polar body (PB) and on the surface of the ZP (Z).d : after Tunicamycin and hyaluronidase treatment, labeling is reduced.e : labeling of control oocyte, similar to : a.f : after hyaluronidase treatment, labeling in PVS (*) is reduced.g : heavy labeling specially of corona cells (C), ZP surface (Z), PVS (*) after Tunicamycin treatment.h : after Tunicamycin and hyaluronidase treatment, labeling of PVS (*) and ZP (Z) surface is reduced.

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