Analysis of cyclin D2 and p27 in ovarian tissue. Equal amounts of total protein from ovaries were resolved on 12.5% SDS-PAGE gels and transferred to nitrocellulose membrane then probed with antibodies described in materials and methods section. Proteins were analyzed from PRLR+/+ and -/- mice at 1.5 and 2.5 d.p.c. Four mice of each genotype were analyzed. Equal amount of total proteins have been loaded as demonstrated by the actin signal. Upper panel, Representative Western blot; lower pane, bars, means ± SEM of the densitometric analysis of cyclinD2 (black bars) and p27 (hatched bars) after normalization of signals against actin.