Expression of P450scc. A. Semi quantitative RT-PCR analysis of P450scc mRNA in +/+ (black bars) and -/- (hatched bars) ovaries at 0.5, 1.5, 2.5 and 3.5 day post coitum (d.p.c.). upper panel, inverse picture of ethidium bromide stained agarose gel shows a typical RT-PCR reaction; lower panel, bars, means ± SEM of the densitometric analysis. B. Western blot analysis of P450scc protein, equal amounts of total proteins were resolved on 10% SDS-PAGE gels and transferred to nitrocellulose membrane then probed with polyclonal antibody at D1.5 and 2.5. C. In situ hybridization of P450scc in ovarian sections at 2.5 d.p.c. of PRLR+/+ and PRLR-/- mice.