Table 1 Oligonucleotides used for the RT-PCR reactions.
From: Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates
Transcript | Number | Orientation | Sequence | Expected Size |
|---|---|---|---|---|
LGR7 | #174 | forward | 5'-TCAACACATGCCAAGACTAC-3' | 522 bp |
LGR7 | #176 | reverse | 5'-GCTTCATTAACTCAGGTGGCATCT-3' | (see Fig. 4C) |
LGR7 | #177 | reverse | 5'-CAAAAACACCCGGCTTCAGGA-3' | Â |
LGR7 | #178 | reverse | 5'-TGCAGATACAACCCAGACA-3' | Â |
LGR7 | #180 | forward | 5'-ATGACATCTGGTTCTGTCTTC-3' | Â |
LGR7 | #181 | reverse | 5'-TCATGAATAGGAATTGAGTCTCG-3' | Â |
LGR7 | #189 | forward | 5'-CGTGTGTGTAAAGAAGGAG-3' | Â |
LGR7 | #191 | forward | 5'-TGTGAGCTGTATGCGATTC-3' | Â |
GAPD | #150 | forward | 5'-TCAGCAATGCCTCCTGCAC-3' | 277 bp |
GAPD | #151 | reverse | 5'-CTGCTTCACCACCTTCTTG-3' | Â |
GAPD | #400 | forward | 5'-GCATCCTGGGCTACACTGAG-3' | 140 bp |
GAPD | #401 | reverse | 5'-ACCACCTGTTGCTGTAGCC-3' | Â |
PRL | Â | forward | 5'-GACAGAGACACCAAGAAGAATCGGAACATA-3' | 875 bp |
PRL | Â | reverse | 5'-GCAATGGAACGGATCATTAAGGACCTTCTC-3' | Â |
IGF-BP1 | Â | forward | 5'-TGCTGCAGAGGCAGGGAGCCC-3' | 378 bp |
IGF-BP1 | Â | reverse | 5'-AGGGATCCTCTTCCCATTCCA-3' | Â |