Figure 3

Amino acid alignment of mature LCN6 and related lipocalins. Lipocalin signature amino acids are in red and cysteines in orange. The predicted casein kinase II phosphorylation sites at S64, T101 and S118 are indicated by dotted underline. Predicted protein kinase A phosphorylation site at Ser73 is magenta and double underlined. Predicted protein kinase C phophorylation sites at S53 and S118 are magenta and single underlined (solid underline for S53 and dotted for S118). Amino acids known to form alpha helices in mouse Mup1 and rat Lcn5 and predicted in LCN6 are green. Beta strands are blue and underlined and labeled A to I corresponding to the β-strands in Fig. 4. The rhesus LCN6 (rhesLCN6) sequence is available at GenBank accession number AF303085. Rat Lcn5 sequence is from accession number X59832 and structural information from 1EPA.pdb and (61). Mouse Lcn8 accession number is NM_033145. Mouse major urinary protein 1 (Mup1) is from M16355. Intron locations in human LCN6 are indicated by the filled triangles (▼). Numbering refers to LCN6 amino acid positions in the mature protein.