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Table 3 Advantages and disadvantages of density gradient centrifugation.

From: Sperm preparation for ART

Advantages Disadvantages
- usually clean fraction of highly motile spermatozoa - production of good interphases between the different media is a bit more time-consuming
- spermatozoa from ejaculates with a very low sperm density can be separated - a bit more expensive
- good yield - potential risk of endotoxins
- leukocytes are eliminated to a large extent - Percoll® may no longer be used IVF/ICSI
- reactive oxygen species are significantly reduced