Open Access

Erratum to: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

  • Amelie Fassbender,
  • Peter Simsa,
  • Cleophas M Kyama,
  • Etienne Waelkens,
  • Attila Mihalyi,
  • Christel Meuleman,
  • Olivier Gevaert,
  • Raf Van de Plas,
  • Bart de Moor and
  • Thomas M D'Hooghe1, 2, 3, 4Email author
Reproductive Biology and Endocrinology20119:44

DOI: 10.1186/1477-7827-9-44

Received: 29 March 2011

Accepted: 6 April 2011

Published: 6 April 2011

The Methodology to this article has been published in Reproductive Biology and Endocrinology 2010 8:123

Since publication of our article [1], we have realised that we did not include the full data in Table 1 and missed the word (TOF) in the result/discussion section. We have provided here the adapted sentence and another version of the table, including all the information intended.
Table 1

The representative molecular weights of the proteins identified in the mRNA Microarray study [Thirteen] [2]

Protein

Mass in Da

Osteoglycin (OGN/4969)

33,922

Interleukin-6 signal transducer (IL6ST/3572)

isoform 1 103,537

isoform 2 37,499

Cytochrome P450, Family 2, Subfamily J, polypeptide 2 (CYP2J2/1573)

57,611

Carboxypeptidase E (CPE/1363)

53,151

Fibronectin 1 (FN1/2335)

different isoforms

 

   1. 262,607

 

   2. 71,943

 

   3. 259,198

 

   4. 222,944

 

   5. 243,316

 

   6. 240,477

 

   7. 268,894

 

   8. 252,793

 

   9. 246,670

 

   10. 239,608

 

   11. 262,388

 

   12. 221,274

 

   13. 249,304

 

   14. 249,384

 

   15. 272,302

Synuclein, gamma (SNCG/6623)

13,331

BAI1-associated protein 2 (BAIAP2/10458)

different isoforms

 

   1. 60,868

 

   2. 59,014

 

   3. 56,626

 

   4. 57,359

 

   5. 57,445

 

   6. 57,430

Protocadherin 17 (PCDH17/27253)

different isoforms

 

   1. 126,229

 

   2. 96,570

Protein tyrosine phosphatase, receptor type, R (PTPRR/5801)

Alpha 73,834 Da

Gamma 46,581Da

Delta 51,046Da

Results/Discussion

"Therefore, we plan to repeat this study in a larger sample size including well defined endometrial samples obtained during menstrual, follicular and secretory phase, to validate the reproducibility of SELDI-TOF MS technology in these samples and to identify the protein peaks observed after proteomic analysis, which are expensive and labour intense requiring High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) and matrix assisted laser desorption ionization Time-of-Flight-Mass Spectrometry (MALDI-TOF/TOF MS)."

Notes

Authors’ Affiliations

(1)
Department of Obstetrics & Gynaecology, Leuven University Fertility Centre,University Hospital Gasthuisberg
(2)
Division of Reproductive Health and Biology, Institute of Primate Research
(3)
Biochemistry Section, Department of Molecular Cell Biology, Campus Gasthuisberg
(4)
Department of Electrical Engineering, ESAT-SCD, K.U.Leuven,Kasteelpark-Arenberg 10

References

  1. Fassbender Amelie, Simsa Peter, Kyama Cleophas, Waelkens Etienne, Mihalyi Attila, Meuleman Christel, Gevaert Olivier, Van de Plas Raf, de Moor Bart, D'Hooghe Thomas: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis. Reproductive Biology and Endocrinology. 2010, 8: 123-10.1186/1477-7827-8-123.PubMed CentralView Article
  2. Sherwin JR, Sharkey AM, Mihalyi A, Simsa P, Catalano RD, D'Hooghe TM: Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis. Hum Reprod. 2008, 23 (5): 1063-1068. 10.1093/humrep/den078.View Article

Copyright

© Fassbender et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement