Erratum to: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

  • Amelie Fassbender,

    Affiliated with

    • Peter Simsa,

      Affiliated with

      • Cleophas M Kyama,

        Affiliated with

        • Etienne Waelkens,

          Affiliated with

          • Attila Mihalyi,

            Affiliated with

            • Christel Meuleman,

              Affiliated with

              • Olivier Gevaert,

                Affiliated with

                • Raf Van de Plas,

                  Affiliated with

                  • Bart de Moor and

                    Affiliated with

                    • Thomas M D'Hooghe1, 2, 3, 4Email author

                      Affiliated with

                      Reproductive Biology and Endocrinology20119:44

                      DOI: 10.1186/1477-7827-9-44

                      Received: 29 March 2011

                      Accepted: 6 April 2011

                      Published: 6 April 2011

                      The original article was published in Reproductive Biology and Endocrinology 2010 8:123

                      Since publication of our article [1], we have realised that we did not include the full data in Table 1 and missed the word (TOF) in the result/discussion section. We have provided here the adapted sentence and another version of the table, including all the information intended.
                      Table 1

                      The representative molecular weights of the proteins identified in the mRNA Microarray study [Thirteen] [2]

                      Protein

                      Mass in Da

                      Osteoglycin (OGN/4969)

                      33,922

                      Interleukin-6 signal transducer (IL6ST/3572)

                      isoform 1 103,537

                      isoform 2 37,499

                      Cytochrome P450, Family 2, Subfamily J, polypeptide 2 (CYP2J2/1573)

                      57,611

                      Carboxypeptidase E (CPE/1363)

                      53,151

                      Fibronectin 1 (FN1/2335)

                      different isoforms

                       

                         1. 262,607

                       

                         2. 71,943

                       

                         3. 259,198

                       

                         4. 222,944

                       

                         5. 243,316

                       

                         6. 240,477

                       

                         7. 268,894

                       

                         8. 252,793

                       

                         9. 246,670

                       

                         10. 239,608

                       

                         11. 262,388

                       

                         12. 221,274

                       

                         13. 249,304

                       

                         14. 249,384

                       

                         15. 272,302

                      Synuclein, gamma (SNCG/6623)

                      13,331

                      BAI1-associated protein 2 (BAIAP2/10458)

                      different isoforms

                       

                         1. 60,868

                       

                         2. 59,014

                       

                         3. 56,626

                       

                         4. 57,359

                       

                         5. 57,445

                       

                         6. 57,430

                      Protocadherin 17 (PCDH17/27253)

                      different isoforms

                       

                         1. 126,229

                       

                         2. 96,570

                      Protein tyrosine phosphatase, receptor type, R (PTPRR/5801)

                      Alpha 73,834 Da

                      Gamma 46,581Da

                      Delta 51,046Da

                      Results/Discussion

                      "Therefore, we plan to repeat this study in a larger sample size including well defined endometrial samples obtained during menstrual, follicular and secretory phase, to validate the reproducibility of SELDI-TOF MS technology in these samples and to identify the protein peaks observed after proteomic analysis, which are expensive and labour intense requiring High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) and matrix assisted laser desorption ionization Time-of-Flight-Mass Spectrometry (MALDI-TOF/TOF MS)."

                      Notes

                      Authors’ Affiliations

                      (1)
                      Department of Obstetrics & Gynaecology, Leuven University Fertility Centre,University Hospital Gasthuisberg
                      (2)
                      Division of Reproductive Health and Biology, Institute of Primate Research
                      (3)
                      Biochemistry Section, Department of Molecular Cell Biology, Campus Gasthuisberg
                      (4)
                      Department of Electrical Engineering, ESAT-SCD, K.U.Leuven,Kasteelpark-Arenberg 10

                      References

                      1. Fassbender Amelie, Simsa Peter, Kyama Cleophas, Waelkens Etienne, Mihalyi Attila, Meuleman Christel, Gevaert Olivier, Van de Plas Raf, de Moor Bart, D'Hooghe Thomas: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis. Reproductive Biology and Endocrinology. 2010, 8: 123-10.1186/1477-7827-8-123.PubMed CentralView Article
                      2. Sherwin JR, Sharkey AM, Mihalyi A, Simsa P, Catalano RD, D'Hooghe TM: Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis. Hum Reprod. 2008, 23 (5): 1063-1068. 10.1093/humrep/den078.View Article

                      Copyright

                      © Fassbender et al; licensee BioMed Central Ltd. 2011

                      This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                      Advertisement