Alpha-defensins 1-3 release by dendritic cells is reduced by estrogen
- Maria M Escribese†1, 2, 3Email author,
- Marta Rodríguez-García†4, 5,
- Rhoda Sperling6,
- Stephanie M Engel7,
- Teresa Gallart4 and
- Thomas M Moran1, 2
© Escribese et al; licensee BioMed Central Ltd. 2011
Received: 16 May 2011
Accepted: 23 August 2011
Published: 23 August 2011
During pregnancy the immune system of the mother must protect any activation that may negatively affect the fetus. Changes in susceptibility to infection as well as resolution of some autoimmune disorders represent empirical evidence for pregnancy related alterations in immunity. Sex hormones reach extremely high levels during pregnancy and have been shown to have direct effects on many immune functions including the antiviral response of dendritic cells. Among the immunologically active proteins secreted by monocyte derived DCs (MDDC) are the alpha-defensins 1-3. This family of cationic antimicrobial peptides has a broad spectrum of microbicidal activity and has also been shown to link innate to adaptive immunity by attracting T cells and immature DCs, which are essential for initiating and polarizing the immune response.
We compare culture-generated monocyte derived DCs (MDDCs) with directly isolated myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) and measure their alpha-defensins 1-3 secretion by ELISA both, in basal situations and after hormone (E2 or PG) treatments. Moreover, using a cohort of pregnant women we isolated mDCs from blood and also measure the levels of these anti-microbial peptides along pregnancy.
We show that mDCs and pDCs constitutively produce alpha-defensins 1-3 and at much higher levels than MDDCs. Alpha-defensins 1-3 production from mDCs and MDDCs but not pDCs is inhibited by E2. PG does not affect alpha-defensins 1-3 in any of the populations. Moreover, alpha-defensins 1-3 production by mDCs was reduced in the later stages of pregnancy in 40% of the patients.
Here, we demonstrate that mDCs and pDCs secrete alpha-defensins 1-3 and present a novel effect of E2 on the secretion of alpha-defensins 1-3 by dendritic cells.
Defensins are a family of cationic antimicrobial peptides  that demonstrate a broad-spectrum of microbicidal activity against bacteria, fungi and viruses . Moreover, recent evidence suggest that defensins also interact with host immune cells playing an important role in both the innate and adaptive immune response . Two groups of human defensins have been described based on their structural characteristics: α- and β-defensins . Both types have antimicrobial activity against bacteria and fungi as well as against enveloped virus such as influenza, herpes virus, cytomegalovirus and HIV . α-defensins 1-3 specifically, are secreted by monocytes, monocyte-derived DCs , NK cells and γδT cells in addition to neutrophils . Moreover, α-defensins 1-3 have been detected throughout the female reproductive tract (FRT) in non-pregnant women [5, 6] as well as in the amnion, chorion, placenta, amniotic fluids and cervical mucus plug from pregnant women [7–9]. Changes in these peptides have also been reported to be involved in several pregnancy complications such as preterm delivery and preeclampsia [7, 10].
Dendritic cells (DCs), as immunological sentinels, are crucial intermediates linking innate immunity to adaptive immunity. Upon exposure to microbial invaders, they undergo a maturational change characterized by the up-regulation of surface molecules involved in the interaction with T cells and the release of numerous cytokines. This culminates in their migration to lymph nodes, where microbe-derived peptides are presented to specific T cells and initiation of the adaptive immune response occurs [11, 12]. Most studies utilize DCs generated from CD14+ monocyte precursors, monocyte derived dendritic cells (MDDCs) cultured in cytokine-supplemented media. However, fully functional DCs can be directly isolated from human blood based upon the surface expression of CD1c+ (mDCs) without the need for cytokine differentiation. Other DC populations such as the plasmacytoid DCs (pDCs) have been proposed as important mediators of innate immunity because of their ability to produce large amounts of type I interferon (IFN) following viral infection.
Steroid sex hormones exert a powerful effect on various immune system functions [13, 14]. The monthly hormonal changes associated with the female menstrual cycle also correlate with deviations in immunity . However, the most drastic changes in immunity occur during pregnancy when the levels of estrogen (E2) and progesterone (PG) rise to extremely high concentrations in the third trimester [16, 17]. Some defensins, such as HD-5 and HBD1-4 undergo variations at mucosal surfaces during the menstrual cycle [6, 18], suggesting that α-defensins 1-3 may be regulated by changes in hormonal levels .
Numerous interactions between the endocrine and the immune systems have been described [20–22]. Several groups have shown that estrogen administration leads to clinical improvement in experimental autoimmune encephalomyelitis due to changes in DCs function and the promotion of a Th2 immune response . In contrast, disease exacerbation has been reported to occur during pregnancy in systemic lupus erythematosus (SLE) patients . Progesterone (PG) also causes a shift to a Th2 dominated response by blocking the Th1 pathway, inducing IL-10 and blocking IL12 [13, 14].
E2 has a wide range of physiological activities, and it is thought to act through two receptors ERα and ERβ by direct and indirect binding to promoter and/or enhancer regions within target genes . Receptors for PG and E2 have been detected on some leukocytes [26, 27] and in particular on DCs . At least three distinct classes of anti-estrogens have been described. In this study we have used a type I antagonists represented by ICI 182,780 that inhibits estrogen activity both in vivo and in vitro. In contrast, type II and III, represented by Tamoxifen and Raloxifene mimic the biological effects of E2 in some specific tissues but oppose E2 action in other .
In this report we demonstrate that directly isolated DCs secrete α-defensins 1-3, and that E2 acts to inhibit this secretion. This same trend is observed in mDCs isolated from pregnant women in their first trimester (low E2) and third trimester (high E2). Together these data suggest a direct effect of E2 over the secretion of α-defensins 1-3 by dendritic cells.
Isolation and culture of human DCs
PBMCs were isolated as previously described . Briefly, Ficoll density gradient centrifugation (Histopaque; Sigma Aldrich) was performed using buffy coats from healthy human donors (Mount Sinai Blood Donor Center and New York Blood Center). CD14+ cells were immunomagnetically purified using anti-human CD14+ antibody-labeled magnetic beads and iron-based Midimacs LS columns (Miltenyi Biotec). After elution from the columns, these cells were plated (0.7 × 106 cells/ml) in DC medium (RPMI [Invitrogen], 1% Human Serum, 100 units/ml of penicillin, and 100 μg/ml streptomycin [Invitrogen]) supplemented with 500 U/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF; Peprotech) and 1,000 U/ml human interleukin-4 (IL-4; Peprotech) and incubated for 5 to 6 days at 37°C. mDC and pDCs were immunologicaly purified using anti-human CD1c (BDCA-1) and CD303 (BDCA-4) antibody-labeled magnetic beads (Miltenyi Biotec) and iron based MiniMacs LS columns. After elution from columns, cells were plated (1 × 106 cells/ml) and treated or not with E2 or PG.
Hormone treatment and viral infection
After 5 to 6 days in culture at a 1 × 106 cell/ml concentration, MDDCs were treated either with 17β-estradiol (E2; Sigma) or with progesterone (PG; Sigma) at concentrations of 10 μg/ml, 1 μg/ml, 0.1 μg/ml and mock (0 ug/ml). In some cases the MDDCs treated for 24 h with 10 ug/ml of E2 or PG were infected with New Castle Disease Virus NDV-B1 for 10 h at and MOI (multiplicity of infection) of 0.5. mDCs and pDCs were treated with each specific condition right after their isolation. After mock or E2/PG treatment supernatants were collected and α-defensins 1-3 secretion was measured by ELISA.
ICI 182,780 treatment
MDDCs at day 2 in the differentiation process in a concentration of 1 × 106 cell/ml are treated with 10, 100 and 1000 nM of either ICI 182,780 (AstraZeneca) or the vehicle (DMSO) for 3 days until day 5 of the differentiation process. mDCs were pretreated with ICI 182,780 for 24 h previous to the hormonal treatment. Cell viability, in both cell populations, was measured before and after treatment by Trypan Blue (Invitrogen) exclusion. After ICI 182,780 pre-treatment DCs were either mock or E2 treated for 24 h and supernatants and cell lysates were collected for further analysis.
Capture enzyme-linked immunosorbent assays (ELISAs) for HNP1-3 (Hycult Biotechnology) was used according to the manufacturers' instructions to quantify the α-defensins 1-3 in the DC supernatants from pregnant women. Plates were read in an ELISA reader from Biotek Instruments assay following the manufacturer's protocol and data were analyzed using software from Applied Cytometry Systems. Levels of serum estradiol and progesterone were measured by Lenetix Medical Screening Laboratory, Inc., Mineola, New York.
Cells were stained with CD14 FITC/CD11cPE (from BD Bioscience), to verify the differentiation process from monocytes to MDDCs. The staining was performed according to the manufacturer's instructions (Beckman Coulter or BD-Pharmingen). The expression level of each marker was determined by flow cytometry after gating on the lineage-specific marker, using a FC 500 flow cytometer from Beckman Coulter. Data were analyzed using CXP software (Beckman Coulter).
RNA extraction and Quantitative real-time PCR from human DCs
RNA extraction and RT-PCR was performed as described before . Briefly, samples of 1 × 106 DCs differentially treated according to the experimental protocol were pelleted, and RNA was isolated and treated with DNase using Absolutely RNA RT-PCR miniprep kit (Stratagene, La Jolla, CA, USA). RNA was quantified using a Nanodrop spectrophotometer (Nanodrop Technologies). The yields of RNA were approximately 20-50 ug/sample. qRT-PCR was performed by using a previously published SYBR green protocol with an ABI7900 HT thermal cycler . Each transcript in each sample was assayed two times, and the mean cycle threshold was used to calculate the fold change for each gene. Four housekeeping genes were used for global normalization in each experiment (actin, Rps11, glyceraldehyde-3-phosphate dehydrogenase, and tubulin genes) . Data validity by modeling of reaction efficiencies and analysis of measurement precision was determined as described previously .
Maternal blood and serum samples utilized in these experiments were obtained from the Viral Immunity in Pregnancy (VIP) study, an ongoing NIH-funded project which enrolls pregnant women during the first 20 weeks of gestation and follows them through 6 months post delivery in order to assess the interplay between maternal systemic immunologic responses and enhanced susceptibility to viral pathogens. The study is IRB approved by the Program for the Protection of Human Subjects at the Mount Sinai School of Medicine. Peripheral blood samples are collected from women at five specific time points: study entry (< 20 weeks' gestation), 25-27 weeks' gestation, 34-36 weeks' gestation, 6 weeks post-delivery and 6 months post-delivery. Each woman's perinatal or 6 weeks postpartum blood draw can be compared against her own 6 month post-partum "normal" blood draw, thus allowing each woman to serve as her own control, implicitly accounting for interindividual variation in a number of factors. Women are recruited from the general obstetrics practice at the Mount Sinai Hospital in NYC. Exclusion criteria include: (1) Individuals with known or suspected HIV infection; (2) Individuals with a history of splenectomy, known auto-immune disorders (systemic lupus erythematosus or rheumatoid arthritis), or require chronic systemic immunosuppressive therapy; (3) Individuals with a history of previous bone marrow or organ transplant; (4) Individuals who have received any immune globulin (including Rhogam) or blood derived products within 3 months of study enrollment; (5) Individuals who became pregnant using a donor egg for this pregnancy; (6) Individuals with a history of malignancy; and, (7) Individuals that will be managed in the third trimester of pregnancy with epogen. Individuals with asthma were eligible to participate as long as they did not receive chronic oral steroid treatment. Individuals with anemia, defined by a Hemoglobin < 10 g/dl were excluded by the local IRB because of safety concerns re: volume of blood donation required by the study.
Data were transformed with natural-logarithms. Mixed models were used to examine changes in defensins during pregnancy accounting for repeated measures taken from the same women over time. The analyses were performed using PROC MIXED of SAS, Version 9.2 (SAS Inc., Cary, NC). All P values were two-tailed, with P < 0.05 considered as statistically significant. Statistical analysis for changes in a-defensins 1-3 induce by hormones treatments was performed by analysis of variance, and a P value less than .05 was considered significant.
Myeloid DCs (mDCs) and plasmacytoid (pDCs) secrete a-defensins 1-3
Estrogen but not Progesterone down-regulates a-defensins 1-3 secretion by DCs
Estrogen exerts an inhibitory effect on mDC and MDDC secretion of type I interferon (IFN) and some inflammatory cytokines . Therefore we measured the effect of estrogen (E2) and progesterone (PG) on α-defensins 1-3 secretion by MDDCs, mDCs and pDCs. Treatment of MDDCs with E2 resulted in a dose-dependent reduction in α-defensin 1-3 secretion. Conversely, PG treatment had no effect on the secretion of α-defensins 1-3 in MDDCs (Figure 1B). Moreover, we also show that E2 but not PG still have a significant (*p < 0.05 vs mock treated DCs) inhibitory effect in the secretion of α-defensins 1-3 in viral infected MDDCs (Figure 1C), that correlates with our previous report describing the immunosuppressive effect of E2 during viral infection .
Effect of ICI 182,780 in the secretion of α-defensins 1-3 by DCs
Secretion of a-defensins 1-3 by DCs during pregnancy
During normal pregnancy, the maternal immune system remains systemically immunocompetent [33, 34]. However, it is faced with a dilemma that it must continue to protect the mother against infection while inhibiting immune activation against the semiallogeneic fetus. There is clear evidence that in the later stages of pregnancy women are uniquely susceptible to microbial infection. The prevailing view, though not shared by all, is that the predominant immune response is shifted from a gamma interferon based Th1 response to a less inflammatory Th2 response. The period of enhanced sensitivity to infection corresponds to the extremely high concentrations of E2 and PG that are reached in the later stages of pregnancy [16, 17]. Both E2 and PG have been shown to exert a powerful effect on various aspects of the innate and adaptive immune response [13, 14]. In a previous publication we investigated the effect of these hormones on the activation of DCs cultured from monocytes as well as those isolated directly from the blood. PG did not exert any effect on DCs in either the steady state or after activation by viruses. In contrast, E2 was a potent inhibitor of DC activation triggered by a number of human viruses but not by toll-like receptors ligands (TLRs) .
Alpha-defensins 1-3 are small peptides that can act both as natural antibiotics by directly killing a wide range of microorganisms and by participating in adaptive immunity by attracting monocytes, T cells, or immature DCs [35, 36]. Moreover, α-defensins 1-3 have been detected throughout the female reproductive tract (FRT) in non-pregnant women [5, 6] as well as in the amnion, chorion, placenta, amniotic fluids and cervical mucus plug from pregnant women [7–9]. Previous reports by us and others demonstrate the expression of α-defensins 1-3 by immune cells populations including MDDCs , NK cell, B cells, γδTcells, mast cells, neutrophils . Most human DC studies are performed using MDDCs produced by culturing these cells in the presence of the cytokines GM-CSF and IL4. It is not known whether cells produced this way simulate in vivo DC populations. However, mDCs as well as pDCs can be isolated directly from human blood by the expression of the CD1c marker (mDC) or BCDA4 (pDC) on their surface . In this report we analyzed directly isolated mDCs and pDCs and show that they both secrete significantly more α-defensins 1-3 than MDDCs. These results suggest that culturing of DCs may reduce their α-defensin 1-3 secretion and that previous work may have underestimated the contribution of DCs to in vivo α-defensin 1-3 production .
17-β-estradiol (E2), one of the most important forms of estrogen, acts through binding to two known hormone receptors, estrogen receptors ERα and ERβ , which are expressed in specific tissues as well as in immune cells, including macrophages, CD8+ T cells, CD4+ T cells, B cells, monocytes, and DCs . Cells pre-treated with ICI 182, 780, a specific ER antagonist, became insensitive to the inhibitory effect of E2, demonstrating a direct and specific effect of E2 on the release of α-defensins 1-3 by DCs. However, intrinsic or subsequently acquired resistance to the therapy remains a major obstacle in treatment with this compound as has been describe by Zhao et al., using a cell line of breast cancer . These open new questions that should be addressed in further investigations with dendritic cells. In our system, ICI 182,780 reduces the expression of the ER, as a result of the induction of a proteasome-dependent degradation induced by this compound . However, ICI 182,780 treatment does not affect the differentiation of monocytes into MDDCs as measured by percentage of CD11c+CD14- cells.
E2 but not PG suppressed the α-defensins 1-3 production from the mDCs at high concentration but had no effect on pDC α-defensins 1-3 secretion. This result is consistent with our earlier observation that E2 did not inhibit activation or cytokine secretion from pDCs. A possible explanation for these observations comes from the work of Hartman et al who demonstrated that murine pDCs derive exclusively from estrogen-resistant myeloid progenitors  which may explain the failure of E2 to alter α-defensin 1-3 production by these cells.
Based upon our in vitro studies we expected that mDCs collected from the blood in the later part of pregnancy would show a reduction in α-defensins 1-3 production. Moreover, evidence suggests that elevated levels of α-defensins 1-3 in serum correlated with adverse pregnancy outcomes [43, 44]. While we observed a trend towards less α-defensins 1-3 production from DCs collected in the 3rd trimester relative to those collected from the same patient in the 1st trimester, the difference was not statistically significant. Almost 40% of the patients did show a very substantial drop in α-defensins 1-3 release from their mDCs at third trimester, while the remaining patients secreted α-defensins 1-3 at nearly identical levels to what was observed in the 1st trimester. It is worth noting, however, that the levels of estrogen detected in serum were lower than the dose that most effectively inhibited α-defensin 1-3 secretion in vitro.
Among the 50% failed to show a reduction, the consistency of the mDC α-defensin 1-3 secretion within patients and variation between patients (ranging from 10 ng/ml to 60 ng/ml) was significant. This high inter-donor variability has been well described and likely corresponds to variations in the copy number of the genes that encode for α-defensins 1-3 between individuals .
The sum of the α-defensin 1-3 production by mDCs suggested a net loss in the later stages of pregnancy. If other cells that produce α-defensins 1-3 were likewise affected, serum α-defensins1-3 during pregnancy would be expected to drop.
In this report we document that myeloid and plasmacytoid DCs directly isolated from the blood produce substantial amounts of α-defensins 1-3 constitutively. pDCs are not affected, but both mDCs and MDDCs produce less α-defensins 1-3 in the presence of high levels of E2. Alpha-defensins 1-3 release from mDCs collected in the third trimester of pregnancy either fell or stayed the same in the majority of the patients.
Our results demonstrate a specific effect for E2 on the secretion of a-defensins 1-3 by dendritic cells. Moreover, we also observed this effect in dendritic cells isolated from pregnant women at third trimester, when the levels of E2 reach extremely high concentrations. Together, this data provide new insights that contribute to explain immune changes during pregnancy.
List of abbreviations
Female reproductive track
Human beta defensins 1-4
Monocyte derived dendritic cells
myeloid dendritic cells
plasmacytoid dendritic cells
Estrogen receptor alpha
Estrogen receptor beta
Peripheral blood mononuclear cells.
MME, RS, SE, SW are supported by NIH contract NO1 - AI 50028. TG is mainly supported mainly by Research Grants FIS2003-1200 and FIS2006-1259 from the Spanish Ministry of Health and MRG is the recipient of a research award ''Emili Letang'' (Hospital Clinic, Barcelona).
- Yang D, Biragyn A, Hoover DM, Lubkowski J, Oppenheim JJ: Multiple roles of antimicrobial defensins, cathelicidins, and eosinophil-derived neurotoxin in host defense. Annu Rev Immunol. 2004, 22: 181-215. 10.1146/annurev.immunol.22.012703.104603.View ArticlePubMed
- Klotman ME, Chang TL: Defensins in innate antiviral immunity. Nat Rev Immunol. 2006, 6 (6): 447-456. 10.1038/nri1860.View ArticlePubMed
- Shi J, Aono S, Lu W, Ouellette AJ, Hu X, Ji Y, Wang L, Lenz S, van Ginkel FW, Liles M, Dykstra C, Morrison EE, Elson CO: A novel role for defensins in intestinal homeostasis: regulation of IL-1beta secretion. J Immunol. 2007, 179 (2): 1245-1253.View ArticlePubMed
- Rodriguez-Garcia M, Oliva H, Climent N, Garcia F, Gatell JM, Gallart T: Human immature monocyte-derived dendritic cells produce and secrete alpha-defensins 1-3. J Leukoc Biol. 2007, 82 (5): 1143-1146. 10.1189/jlb.0507295.View ArticlePubMed
- King AE, Critchley HO, Kelly RW: Innate immune defences in the human endometrium. Reprod Biol Endocrinol. 2003, 1: 116-10.1186/1477-7827-1-116.PubMed CentralView ArticlePubMed
- King AE, Fleming DC, Critchley HO, Kelly RW: Differential expression of the natural antimicrobials, beta-defensins 3 and 4, in human endometrium. J Reprod Immunol. 2003, 59 (1): 1-16. 10.1016/S0165-0378(02)00083-9.View ArticlePubMed
- King AE, Kelly RW, Sallenave JM, Bocking AD, Challis JR: Innate immune defences in the human uterus during pregnancy. Placenta. 2007, 28 (11-12): 1099-1106. 10.1016/j.placenta.2007.06.002.View ArticlePubMed
- Cole AM: Innate host defense of human vaginal and cervical mucosae. Curr Top Microbiol Immunol. 2006, 306: 199-230. 10.1007/3-540-29916-5_8.PubMed
- Quayle AJ: The innate and early immune response to pathogen challenge in the female genital tract and the pivotal role of epithelial cells. J Reprod Immunol. 2002, 57 (1-2): 61-79. 10.1016/S0165-0378(02)00019-0.View ArticlePubMed
- King AE, Paltoo A, Kelly RW, Sallenave JM, Bocking AD, Challis JR: Expression of natural antimicrobials by human placenta and fetal membranes. Placenta. 2007, 28 (2-3): 161-169. 10.1016/j.placenta.2006.01.006.View ArticlePubMed
- van Duivenvoorde LM, van Mierlo GJ, Boonman ZF, Toes RE: Dendritic cells: vehicles for tolerance induction and prevention of autoimmune diseases. Immunobiology. 2006, 211 (6-8): 627-632. 10.1016/j.imbio.2006.05.014.View ArticlePubMed
- Granucci F, Zanoni I, Feau S, Capuano G, Ricciardi-Castagnoli P: The regulatory role of dendritic cells in the immune response. Int Arch Allergy Immunol. 2004, 134 (3): 179-185. 10.1159/000078764.View ArticlePubMed
- Orsi NM, Tribe RM: Cytokine networks and the regulation of uterine function in pregnancy and parturition. J Neuroendocrinol. 2008, 20 (4): 462-469. 10.1111/j.1365-2826.2008.01668.x.View ArticlePubMed
- Beagley KW, Gockel CM: Regulation of innate and adaptive immunity by the female sex hormones oestradiol and progesterone. FEMS Immunol Med Microbiol. 2003, 38 (1): 13-22. 10.1016/S0928-8244(03)00202-5.View ArticlePubMed
- Arruvito L, Sanz M, Banham AH, Fainboim L: Expansion of CD4+CD25+and FOXP3+ regulatory T cells during the follicular phase of the menstrual cycle: implications for human reproduction. J Immunol. 2007, 178 (4): 2572-2578.View ArticlePubMed
- Bukovsky A, Cekanova M, Caudle MR, Wimalasena J, Foster JS, Henley DC, Elder RF: Expression and localization of estrogen receptor-alpha protein in normal and abnormal term placentae and stimulation of trophoblast differentiation by estradiol. Reprod Biol Endocrinol. 2003, 1: 13-10.1186/1477-7827-1-13.PubMed CentralView ArticlePubMed
- Edelstam G, Karlsson C, Westgren M, Lowbeer C, Swahn ML: Human chorionic gonadatropin (hCG) during third trimester pregnancy. Scand J Clin Lab Invest. 2007, 67 (5): 519-525. 10.1080/00365510601187765.View ArticlePubMed
- Quayle AJ, Porter EM, Nussbaum AA, Wang YM, Brabec C, Yip KP, Mok SC: Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. Am J Pathol. 1998, 152 (5): 1247-1258.PubMed CentralPubMed
- Schaefer TM, Wright JA, Pioli PA, Wira CR: IL-1beta-mediated proinflammatory responses are inhibited by estradiol via down-regulation of IL-1 receptor type I in uterine epithelial cells. J Immunol. 2005, 175 (10): 6509-6516.View ArticlePubMed
- Daynes RA, Araneo BA, Hennebold J, Enioutina E, Mu HH: Steroids as regulators of the mammalian immune response. J Invest Dermatol. 1995, 105 (1 Suppl): 14S-19S.View ArticlePubMed
- Khardori R, Adamski A, Khardori N: Infection, immunity, and hormones/endocrine interactions. Infect Dis Clin North Am. 2007, 21 (3): 601-615, vii. 10.1016/j.idc.2007.06.002.View ArticlePubMed
- Lang TJ: Estrogen as an immunomodulator. Clin Immunol. 2004, 113 (3): 224-230. 10.1016/j.clim.2004.05.011.View ArticlePubMed
- Pettersson A, Ciumas C, Chirsky V, Link H, Huang YM, Xiao BG: Dendritic cells exposed to estrogen in vitro exhibit therapeutic effects in ongoing experimental allergic encephalomyelitis. J Neuroimmunol. 2004, 156 (1-2): 58-65. 10.1016/j.jneuroim.2004.07.004.View ArticlePubMed
- Carmona F, Font J, Cervera R, Munoz F, Cararach V, Balasch J: Obstetrical outcome of pregnancy in patients with systemic Lupus erythematosus. A study of 60 cases. Eur J Obstet Gynecol Reprod Biol. 1999, 83 (2): 137-142. 10.1016/S0301-2115(98)00312-1.View ArticlePubMed
- Nicholson RI, Johnston SR: Endocrine therapy--current benefits and limitations. Breast Cancer Res Treat. 2005, 93 (Suppl 1): S3-10.View ArticlePubMed
- Olsen NJ, Kovacs WJ: Gonadal steroids and immunity. Endocr Rev. 1996, 17 (4): 369-384.PubMed
- Cutolo M, Sulli A, Seriolo B, Accardo S, Masi AT: Estrogens, the immune response and autoimmunity. Clin Exp Rheumatol. 1995, 13 (2): 217-226.PubMed
- Escribese MM, Kraus T, Rhee E, Fernandez-Sesma A, Lopez CB, Moran TM: Estrogen inhibits dendritic cell maturation to RNA viruses. Blood. 2008, 112 (12): 4574-84. 10.1182/blood-2008-04-148692.PubMed CentralView ArticlePubMed
- Hall JM, Couse JF, Korach KS: The multifaceted mechanisms of estradiol and estrogen receptor signaling. J Biol Chem. 2001, 276 (40): 36869-36872. 10.1074/jbc.R100029200.View ArticlePubMed
- Fernandez-Sesma A, Marukian S, Ebersole BJ, Kaminski D, Park MS, Yuen T, Sealfon SC, Garcia-Sastre A, Moran TM: Influenza virus evades innate and adaptive immunity via the NS1 protein. J Virol. 2006, 80 (13): 6295-6304. 10.1128/JVI.02381-05.PubMed CentralView ArticlePubMed
- Gonzalez-Maeso J, Yuen T, Ebersole BJ, Wurmbach E, Lira A, Zhou M, Weisstaub N, Hen R, Gingrich JA, Sealfon SC: Transcriptome fingerprints distinguish hallucinogenic and nonhallucinogenic 5-hydroxytryptamine 2A receptor agonist effects in mouse somatosensory cortex. J Neurosci. 2003, 23 (26): 8836-8843.PubMed
- Yang D, Chen Q, Chertov O, Oppenheim JJ: Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells. J Leukoc Biol. 2000, 68 (1): 9-14.PubMed
- Jilani N, Das BC, Husain SA, Baweja UK, Chattopadhya D, Gupta RK, Sardana S, Kar P: Hepatitis E virus infection and fulminant hepatic failure during pregnancy. Journal of gastroenterology and hepatology. 2007, 22 (5): 676-682. 10.1111/j.1440-1746.2007.04913.x.View ArticlePubMed
- Mullooly JP, Barker WH, Nolan TF: Risk of acute respiratory disease among pregnant women during influenza A epidemics. Public Health Rep. 1986, 101 (2): 205-211.PubMed CentralPubMed
- Rodríguez-García MOH, Climent N, Escribese MM, García F, Moran TM, Gatell JM, Gallart T: Impact of alpha-defensins1-3 on the maturation and differentiation of human monocyte-derived DCs. Concentration-dependent opposite dual effects. Clin Immunol. 2009, 131 (3): 374-384. 10.1016/j.clim.2009.01.012.View ArticlePubMed
- Chang TL, Vargas J, DelPortillo A, Klotman ME: Dual role of alpha-defensin-1 in anti-HIV-1 innate immunity. J Clin Invest. 2005, 115 (3): 765-773.PubMed CentralView ArticlePubMed
- Osugi Y, Vuckovic S, Hart DN: Myeloid blood CD11c(+) dendritic cells and monocyte-derived dendritic cells differ in their ability to stimulate T lymphocytes. Blood. 2002, 100 (8): 2858-2866. 10.1182/blood.V100.8.2858.View ArticlePubMed
- Rodriguez-Garcia M, Climent N, Oliva H, Casanova V, Franco R, Leon A, Gatell JM, Garcia F, Gallart T: Increased alpha-defensins 1-3 production by dendritic cells in HIV-infected individuals is associated with slower disease progression. PloS one. 5 (2): e9436-
- Cvoro A, Tatomer D, Tee MK, Zogovic T, Harris HA, Leitman DC: Selective estrogen receptor-beta agonists repress transcription of proinflammatory genes. J Immunol. 2008, 180 (1): 630-636.View ArticlePubMed
- Zhao H, Lo YH, Yu L, Wang SC: Overcoming resistance to fulvestrant (ICI182,780) by downregulating the c-ABL proto-oncogene in breast cancer. Molecular carcinogenesis. 2011, 50 (5): 383-389. 10.1002/mc.20721.View ArticlePubMed
- Lanvin O, Bianco S, Kersual N, Chalbos D, Vanacker JM: Potentiation of ICI182,780 (Fulvestrant)-induced estrogen receptor-alpha degradation by the estrogen receptor-related receptor-alpha inverse agonist XCT790. J Biol Chem. 2007, 282 (39): 28328-28334. 10.1074/jbc.M704295200.View ArticlePubMed
- Harman BC, Miller JP, Nikbakht N, Gerstein R, Allman D: Mouse plasmacytoid dendritic cells derive exclusively from estrogen-resistant myeloid progenitors. Blood. 2006, 108 (3): 878-885. 10.1182/blood-2005-11-4545.PubMed CentralView ArticlePubMed
- Okazaki T, Ota Y, Yuki N, Hayashida A, Shoda A, Nishikawa M, Oshima K, Fukasawa I, Watanabe H, Inaba N: Plasma levels of alpha-defensins 1-3 are an indicator of neutrophil activation in pregnant and post-partum women. J Obstet Gynaecol Res. 2007, 33 (5): 645-650. 10.1111/j.1447-0756.2007.00626.x.View ArticlePubMed
- Prieto JA, Panyutich AV, Heine RP: Neutrophil activation in preeclampsia. Are defensins and lactoferrin elevated in preeclamptic patients?. J Reprod Med. 1997, 42 (1): 29-32.PubMed
- Aldred PM, Hollox EJ, Armour JA: Copy number polymorphism and expression level variation of the human alpha-defensin genes DEFA1 and DEFA3. Human molecular genetics. 2005, 14 (14): 2045-2052. 10.1093/hmg/ddi209.View ArticlePubMed
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